Procedure guides on inside lid Nitrogen in Wastewater When a treatment plant uses processes like nitrication and denitrication, it is important to monitor and maintain the equilibrium between ammonia nitrogen, nitrate and total nitrogen during the bio-treatment. The nitrogen level is important because it relates to the quantity of oxygen provided in the nitrication area. Ammonia is also controlled because it can become very toxic for the bacteria responsible for denitrication. 6 Reasons to Choose Hanna 1. It follows the ISO 23697-2 method 2. Easiest and quickest method 3. Representative sample: 2 mL 4. Exceptional savings 5. Two Ranges available (Low and High) 6. Less interference from chlorides, none from the COD Specifications HI93767A-50 HI93767B-50 Range 0.0 to 25.0 mg/L (as N) 10 to 150 mg/L (as N) Resolution 0.1 mg/L 1 mg/L Accuracy ±1.0 mg/L or ±5% of reading at 25 °C, whichever is greater ±3 mg/L or ±4% of reading at 25 °C, whichever is greater Wavelength 420 nm 420 nm Cuvette Type 13 mm diameter 13 mm diameter Method Chromotropic Acid Method Chromotropic Acid Method Method ID #060 #061 # of Vials 50 50 HI93767A-50 • HI93767B-50 Total Nitrogen Pre-dosed Reagent Set 13 mm Vial Hanna oers two dierent kits of pre-dosed reagents for the analysis of Total Nitrogen: • HI93767A-50 Low Range • HI93767B-50 High Range All kits have vials for 49 tests to be used with Hanna’s HI801 and HI802 spectrophotometers. These high-quality reagents are manufactured in our state-of-the-art facility and are clearly marked with lot number and expiration date for traceability. The method is compliant with ISO 23697-2. The procedure is very simple and fast, with a representative sample volume (2 mL) and it has very few interferences from other substances in the sample (few interferences from chloride, none from COD). The use of 13 mm cuvettes (internal diameter), with a longer optical path than many other vials on the market, ensures accurate absorbance measurements and greater repeatability of results. Signcance and Use Total Nitrogen is the sum of all forms of nitrogen (Nitric nitrogen, Ammonia nitrogen, Nitrous nitrogen and Organic nitrogen). Organic nitrogen includes amino acids found in all living matter. In order to measure organically bound nitrogen the sample has to be digested with acid and heat to convert to nitrate that reacts with chromotropic acid to produce a color proportional to concentration. Total nitrogen is a common wastewater parameter since nitrogen is a nutrient that aects biological growth. Application Wastewater, surface water, groundwater Method The Chromotropic Acid Method is compliant with ISO 23697 Nitrication NH® + O± NO¯± + 3H+ + 2e¯ NO¯± + H±O NO¯® + 2H+ + 2e¯ Denitrication 3NO®¯ + 10e¯ + 12H+ N± + 6H±O nitrosomonas bacteria nitrobacter bacteria 11 COD and Vials 11.30 | www.hannainst.com Pre-dosed Reagents
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