HI93758A-50 • HI93758B-50 • HI93758C-50 HI93763A-50 • HI93763B-50 Phosphorous Pre-dosed Reagent Set 13 mm Vial Hanna oers dierent kits for the analysis of Phosphorous and Phosphate: • HI93758A-50 Orthophosphate Low Range • HI93763A-50 Orthophosphate High Range • HI93758B-50 Polyphosphate Low Range • HI93758C-50 Total Phosphorous Low Range • HI93763B-50 Total Phosphorous High Range Each kit contains vials for 50 tests to be used with Hanna’s HI801 and HI802 spectrophotometers. These high-quality reagents are manufactured in our state-ofthe-art facility and are clearly marked with lot number and expiration date for traceability. The procedure involves the addition of a very representative sample volume (5 mL) and the use of 13 mm (internal diameter) cuvettes, with a longer optical path than many other vials on the market. This ensures accurate absorbance measurements and greater repeatability of results. Signicance and Use Phosphorus is measured during both biological and chemical dephosphorization. An excessive amount of phosphate discharged in supercial waters or in bio-treatment tanks causes an increase of algae and system eutrophication. Orthophosphate (Reactive Phosphorous) Reactive Phosphorous is the simplest form and is known as phosphate or orthophosphate. It is considered reactive since it easily reacts or bonds with cations. Orthophosphate is commonly added to water to inhibit corrosion of pipes in the distribution of water. Polyphosphate (Acid Hydrolyzable Phosphorous) Acid Hydrolyzable Phosphorous also known, as condensed phosphate is a complex form of orthophosphate that are bound together. These forms include polyphosphate, pyrophosphate and metaphosphate, which are used boiler and cooling tower water treatment for corrosion inhibition of pipes. Total Phosphorous Total Phosphorous (Total P) is the sum of all phosphorous including inorganic (orthophosphate and acid hydrolyzable) and organic matter such as the phosphorous found in living matter (i.e. ATP/ADP). In order to measure the organic phosphorous the sample needs to be digested with an acid and heat in order to breakdown the organically bound phosphorous to the simplest form, orthophosphate. It is seen that there are dierent forms of phosphate measurement and it is important to use the correct chemistry for the appropriate one to be measured. Phosphorus is a common parameter measured in wastewater treatment since it can cause eutrophication leading to algal blooms in water. Application Wastewater, environmental monitoring of surface waters and groundwater, Method The method used for Low Range Vials is an Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th Edition, 4500-P E, Ascorbic Acid Method. The method for High Range Vials is Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th Edition, 4500-P C, Vanadomolybdophosphoric Acid Method Procedure guides on inside lid 3 Reasons to Choose Hanna 1. Representative sample: 5 ml 2. Exceptional precision, especially on low values 3. With a single vial, it is possible to determine both Orthophosphates and Total Phosphorus HI93763-50 11 COD and Vials 11.32 | www.hannainst.com Pre-dosed Reagents
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